FIG. 10.
Southern blot of β3 and Δβ3 products from rat tissues following manipulation of thyroid status, hybridized to an exon A internal probe. The 767-bp product is β3, and the lower, 452-bp band is Δβ3. Expression in the pituitary is shown in the lower left panel using RNA from three to five glands of euthyroid (Eu), hypothyroid (Hypo), and thyrotoxic (Tox) rats. Lanes containing genomic DNA (Gen), water (H2O), and the absence or inclusion of reverse transcriptase (RT) (−) or (+) are shown. The upper panels show data from a representative experiment, and the graph represents mean values from three animals (with standard error shown). Graphed data are expressed as the relative ratio of β3 to Δβ3; predominant expression of β3 is in the upward direction, and that of Δβ3 is downward.