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RT-PCR using forward primers from exon A (common to β3 and Δβ3) and TRβ reverse primers (from the second common TRβ exon) to show β3 (767 bp) and Δβ3 (452 bp) mRNA expression in the spleen, heart, and kidneys. Genomic DNA, H2O, or RNA template lacking reverse transcriptase (−) negative controls confirm that expression is dependent on RT (+) and processing of the primary transcript.
