Skip to main content
. 2023 May 15;120(21):e2220684120. doi: 10.1073/pnas.2220684120

Fig. 2.

Fig. 2.

Establishment and characterization of 5xFAD mouse strains with inducible IR KO in astrocytes (iGIRKO/5FAD). (A) Schematic of creation of the iGIRKO/5xFAD mouse. (B) Measurement of body weight (g) at 6-mo-old in male mice. N = 23 to 30 mice per condition. (C and D) 4-h fasting glucose (C) and serum insulin levels (D) in 6-mo-old male mice. N = 20 to 21 mice per condition in C and N = 13 to 21 per condition in D. (E) qPCR analysis of astrocyte marker genes Gfap and Apoe in the cortex of 8-mo-old male mice. N = 5 to 14 mice per condition. (F) Quantification of insulin receptor β-subunit (IRβ) in protein extracted from the cortex of 8-mo-old male mice. N = 4 to 6 per condition. Related to SI Appendix, Fig. S2 C and D. (G) Analysis of scores in nest building test in 6-mo-old male mice (N = 12 to 17 mice per condition). (H) Percent of time with freezing behavior in contextual fear conditioning test in 6-mo-old male mice (N = 19 to 20 mice per condition). *P < 0.05, **P < 0.01, ***P < 0.001, by two-way ANOVA analysis followed by Tukey’s multiple comparisons test. Data are presented as mean ± SEM. Schematic was created with BioRender.