Fig. 5.
Effect of Aβ on insulin signaling and insulin signaling on uptake of Aβ peptides by astrocytes. (A) MFI analysis of Aβ1-42 HiLyte Fluor 647 labeled uptake by sorted neonatal astrocytes (IRf/f) following a 1-h incubation. N = 5 to 6 per condition. (B and C) FACS analysis of Aβ1-42 HiLyte Fluor 647 labeled peptide (B) and Aβ1-40 HiLyte Fluor 647 labeled uptake by isolated adult astrocytes following a 2-h incubation (C). (D) Representative western blots showing the effects of Aβ1-42 (2 µM) on insulin (10 nM)-stimulated signaling pathway. (E) Quantification of insulin signaling molecules and their phosphorylation. N = 3 per condition. *P < 0.05, **P < 0.01, ***P < 0.001 by unpaired t test. Data are presented as mean ± SEM.