Fig. 1.

IRIS: A big-data informed computational platform for discovering AS-derived cancer immunotherapy targets. Overall workflow of IRIS, computational modules, large-scale reference database of AS profiles, and screening tests are illustrated. IRIS has three main computational modules: (A) RNA-seq data processing, (B) in silico screening, and (C) TCR/CAR-T target prediction. A flowchart illustrates the key components and analytical steps of IRIS. (D) Illustration of IRIS DB, a reference database of AS profiles across tumor and normal tissue samples, and two screening tests to assess tumor association and specificity. AS, alternative splicing; TCR, T cell receptor; CAR-T, chimeric antigen receptor T cell.