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. 2023 May 16;120(21):e2221116120. doi: 10.1073/pnas.2221116120

Fig. 4.

Fig. 4.

Evaluation and visualization of IRIS-predicted targets for NEPC. (A) The target evaluation process for NEPC. The three-dimensional scatterplot illustrates the three main criteria used to evaluate IRIS-predicted targets: degree of tumor association, FC of the tumor-enriched isoform between tumor and normal tissues, and gene expression level in tumor tissues. These and additional criteria to evaluate targets are listed below the scatterplot. Criteria illustrated in the scatterplot are bolded. (B) Representative examples of 10 IRIS-predicted TCR targets are visualized by IRIS in paired violin and bar plots. Each row shows one IRIS-predicted TCR target. Violin plots show the PSI values of each target in NEPC and the normal tissue panel. Bar plots show the fraction of samples expressing the SJ(s) of the tumor-enriched isoform in NEPC and the normal tissue panel. If the tumor-enriched isoform is the exon inclusion isoform, the bar plot displays the upstream and downstream inclusion SJ as two bars. If the tumor-enriched isoform is the exon skipping isoform, the bar plot displays the skipping SJ as one bar.