Fig. 1.
Renal ischemia is associated with chronic hypoxia in sickle cell trait mouse model. (A) Schematic of genetically engineered mouse model (GEMM) of SCT. (B) 3D image reconstruction of renal epithelia (RFP) and FITC-dextran (GFP) in adult mice (n = 4 to 5) with kidney-specific CDH16Cre and conditional R26LSL-Tom (C and D) Quantification of the diameter (C) and length (D) of the renal blood vessels (10 vessels/image, 3 locations/vessel). (E) IHC of mouse kidneys after injection with Hypoxyprobe. (F) Quantification of the optical density of horseradish peroxidase (HRP) staining for 20× images was done using ImageJ. Data are expressed as mean value ±SD, with the P value calculated by Student’s t test.