Fig. 4.

SMARCB1-deficient tumors are resistant to hypoxic stress, while SMARCB1-proficient tumors are sensitive to hypoxic stress. (A) Western blotting analysis of MSRT1 cells overexpressed with either SMARCB1^WT or SMARCB1^K62R. (B) Clonogenic assay of MSRT1 tumors overexpressed with either SMARCB1^Null, SMARCB1^WT, and SMARCB1^K62R grown in either normoxia or hypoxia. (C) Representative images of β-galactosidase staining of MSRT1 cells overexpressed with SMARCB1^Null, SMARCB1^WT, and SMARCB1^K62R. (D) Quantification of clonogenic assay. Crystal violet was dissolved from cells using 10% acetic acid. (E) Quantification of β-galactosidase using FIJI ImageJ software. (F) Luminescent (RLU) activity indicating cell viability in MSRT1 with SMARCB1^Null, SMARCB1^WT, and SMARCB1^K62R after prolonged exposure to normoxia and hypoxia. For all quantification experiments shown in D–F, the cell viability of cells in hypoxic condition was normalized to its corresponding normoxic condition. Data are expressed as mean value ±SD, with the P value calculated by Student’s t test.