Fig. 2.

Ginsenoside CK augments mitochondrial fusion and inhibits mitophagy through Mfn2 in the OGD/R-induced PC12 injury model. (A) After labeling with a Mito-tracker probe, mitochondrial morphology was visualized to analyze mitochondria aspect ratio; scale bar = 20 μm. (B) The expression of LC3-II / LC3-II and p62 was detected by western blot. (C) Analysis of the co-location of the GFP-RFP-LC3 fusion protein by confocal microscope; Hochest 33254 was used for nuclear staining; scale bar = 10 μm. (D) The co-localization of Parkin and TOM20 was detected and analyzed; DAPI was used for staining nucleus. Scale bar = 5 μm. (E) The co-location of mitochondria and lysosomes was visualized by confocal microscope; Hochest 33254 was used for nuclear staining; scale bar = 5 μm. Data are shown as mean ± SD, n = 3 per group; ∗P < 0.05, significantly different as indicated (one-way ANOVA followed by Tukey's post hoc test).