Fig. 6.

Ginsenoside CK inhibits the activity of Mul1 and ubiquitination of Mfn2 in the I/ R rat model. (A) The protein expressions of Mul1, Mfn2 and DRP1 in the cytoplasm or mitochondria in rat brain tissues were detected by western blot. (B) The expression of Mfn2 protein in cortex (scale bar = 200 μm) and CA1 region (scale bar = 20 μm) was detected by immunofluorescence assay. (C) The expression of ubiquitin-protein and Mfn2 protein in brain tissues were detected by immunohistochemical staining; scale bar = 20 μm. (D) After the immunoprecipitation of Mul1 or Mfn2 antibody, the binding of Mul1 and Mfn2 and Mfn2 ubiquitination in fresh brain tissues were analyzed by co-IP and Western blot. Ub: ubiquitination, n = 5 per group.