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. 2023 Apr 1;26(5):106555. doi: 10.1016/j.isci.2023.106555

Figure 4.

Figure 4

Glucocorticoid responsive elements (GREs) are located in intronic and conserved regions of ZBTB16 in human β-cells

(A) Distribution of predicted GREs across ZBTB16 gene on the human islets.

(B) Predicted GRE overlaps (and their genomic coordinates) visualized with regulatory/conservation tracks from UCSC Genome Browser; tracks represent from top to bottom: ZBTB16 transcript localization, ENCODE regulatory elements, GeneHancer regulatory elements, Open chromatin positions (DNase I peak clusters), Phylop basewise evolutionary/conservation scores. Red lines on the peak boxes (positions 5, 6, 8, and 10) correspond to GR binding sequence (GBS) occurrences.

(C) Glucocorticoid Receptor ChIP followed by PCR for the predicted GRE coordinates. Position numbers correspond to the positions of (A) from left to right (see also Figure S1). Ctrl; Control (DMSO), Dexa; Dexamethasone (100 nM).