Figure 7.

ZBTB16 predicted direct gene targets are involved in several regulatory pathways, including mitochondrial function

(A) Schematic summary of the bioinformatics workflow to identify ZBTB16 predicted direct gene targets in human pancreatic islets.

(B) Subset of potential islet ZBTB16 targets expressed in human pancreatic β-cells.

(C) Bar chart showing selected enriched terms/pathways derived from the functional annotation of the ZBTB16 predicted direct gene targets in human islets (Up) and human β-cells (Down).

(D) Mitochondrial oxygen consumption measurements in EndoC-βH1 cells (n = 6 biological replicates) corresponding to cellular respiration levels comparing control cells (pcControl + Ctrl), control cells under dexamethasone treatment (pcControl + Dexa) and cells with overexpression of ZBTB16 under dexamethasone treatment (ZBTB16_OE + Dexa), (E) Quantification of baseline OCR levels, (F) Acute response measured as increase in OCR upon pyruvate injection, (G) Maximal respiration measured after addition of an uncoupler of the inner mitochondrial membrane (FCCP), (H) ATP production assessed as decrease in OCR after oligomycin injection, which inhibits ATP-synthase and (I) Coupling efficiency calculated as ATP production in relation to combined baseline and pyruvate response. Comparisons between conditions in each measurement were performed with one-way ANOVA followed by pairwise comparisons with Tukey’s multiple comparison test. pcControl; Control plasmid pcDNA3.1, ZBTB16_OE; ZBTB16 overexpression, Ctrl; Control (DMSO), Dexa; Dexamethasone (100 nM), FCCP; carbonyl cyanide p-trifluoro-methoxyphenyl hydrazone. Data are presented as mean ± SEM; ∗p < 0.05.