REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
ZBTB16 (PLZF) (D8G3G) Rabbit mAb	Cell Signaling Technology	#39784
Anti-rabbit IgG, HRP-linked	Cell Signaling Technology	#7074
Glucocorticoid Receptor (D6H2L) XP® Rabbit mAb	Cell Signaling Technology	#12041
Biological samples
Human pancreatic islets from healthy donors and donors with T2D	Nordic Network for Clinical Islet Transplantation, Human Tissue Laboratory, EXODIAB/LUDC (http://www.nordicislets.org).	N/A
Chemicals, peptides, and recombinant proteins
Dexamethasone	Sigma-Aldrich	#D1756
ZBTB16 TaqMan® gene expression assay	Thermo Fisher	Hs00232313_m1
GR TaqMan® gene expression assay	Thermo Fisher	Hs00353740_m1
SGK1 TaqMan® gene expression assay	Thermo Fisher	Hs00353740_m1
PDX-1 TaqMan® gene expression assay	Thermo Fisher	Hs00236830_m1
INS TaqMan® gene expression assay	Thermo Fisher	Hs02741908_m1
4–15% Mini-PROTEAN® TGX Stain-Free™ Protein Gels	Bio-Rad	#4568084
Clarity Western ECL Substrate	Bio-Rad	#1705061
GelRed® Nucleic Acid Stain	Sigma-Aldrich	#SCT123
Lipofectamine LTX	Thermo Fisher	#15338-100
Lipofectamine™ RNAiMAX Transfection Reagent	Thermo Fisher	#13778075
3-Isobutyl-1-methylxanthine (IBMX)	Sigma-Aldrich	#I5879
Dimethyl sulfoxide (DMSO)	Sigma-Aldrich	#34869
Sodium pyruvate	Sigma-Aldrich	#P2256
Oligomycin A	Sigma-Aldrich	#75351
Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP)	Sigma-Aldrich	#C2920
Rotenone	Sigma-Aldrich	#R8875
Critical commercial assays
miRNeasy isolation kit	Qiagen	#217084
TruSeq Stranded Total RNA Library Prep with Ribo-Zero kit	Illumina	#RS-122-2201
High-Capacity cDNA Reverse Transcription Kit	Thermo Fisher	#4368814
Nextseq 500/550 High Output kit v2	Illumina	#FC-404-2002
Pierce™ BCA Protein Assay Kit	Thermo Fisher	#23225
ChIP-IT® Express Enzymatic kit	Active Motif	#53009
Insulin ELISA (Human)	Mercodia	#10-1113-01
CellTiter 96® AQueous One Solution Cell Proliferation Assay	Promega	#G3582
Deposited data
Raw and processed RNA sequencing data from human islet and EndoC-βH1 cells upon dexamethasone treatment	This paper	GEO: GSE225901
Experimental models: Cell lines
EndoC-βΗ1	Ravassard et al.88	RRID:CVCL_L909
Oligonucleotides
Primers for GR binding sites on ZBTB16 gene, see Table S7	This paper	N/A
ZBTB16 Silencer Select Pre-Designed siRNA	Thermo Fisher	n341241; #4390771
Silencer Select Negative Control No. 2 siRNA	Thermo Fisher	#AM4613
Recombinant DNA
ZBTB16_OHu04229C_pcDNA3.1(+)-C-HA	GeneScript	#SC1200
Software and algorithms
Adobe Illustrator (23.0.1)	Adobe	https://www.adobe.com/products/illustrator.html, RRID:SCR_010279
FastQC (0.11.8)	Andrews et al.89	RRID:SCR_014583
Salmon (0.14.0)	Patro at al.90	RRID:SCR_017036
Deseq2 (1.30)	Love et al.91	RRID:SCR_015687
GraphPad Prism 9	http://www.graphpad.com/	RRID:SCR_002798
WebGestaltR (0.4.4)	Liao et al.92	RRID:SCR_006786
Image Lab Software (6.1)	https://www.bio-rad.com/en-se/product/image-lab-software?ID=KRE6P5E8Z	RRID:SCR_014210