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. 2023 May 26;13:8551. doi: 10.1038/s41598-023-35632-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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No detection of L-OAS from the media of A549 cells transfected with DcsE-FLAG-GFP or FLAG-GFP. (a) Chromatogram of suspected L-OAS (148 m/z) detected from FLAG-GFP (red) and DcsE-FLAG-GFP (black) transfection media. (b, c) ESI-TOF peaks of 148.058 m/z representing suspected L-OAS at 1.14 min from FLAG-GFP (b) and DcsE-FLAG-GFP (c) transfection media. (d,e) Tandem MS of 148 m/z from (b) and (c), respectively, show fragments of 84.0444, 102.0545, 130.0497, and 148.06 m/z at 5 V. The expected 88.0396 and 106.05 fragment ions from an L-OAS standard were not detected.