Table 3.

Cycling conditions for gradient PCR. Reactions were run as needed, and ranges of annealing temperature were tested (see table 3 for annealing temperatures used).

#	Purpose	°C	sec
1	Initial Denaturing	98	30
2	Denaturing	98	10
3	Annealing	69	15
4	Extension	72	Variable
5	Return to step 2 30 times
6	Final extension	72	600