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. Author manuscript; available in PMC: 2023 May 26.
Published in final edited form as: Nature. 2022 May 18;605(7911):701–705. doi: 10.1038/s41586-022-04742-w

Extended Data Fig. 2. Schematics of experimental strategies for reporter assays and Ciona Hmx CNE identification.

Extended Data Fig. 2

a. Hmx overexpression in Ciona. b. Hmx or Ngn CRISPR Cas9 knockout in Ciona. c. Ciona Hmx CNE identification. Approximately 2Kbp 5’ to the first Hmx exon in Ciona intestinalis (Type A) (Ciona robusta) scaffold KhS563 is shown, with conservation to the Hmx locus in Ciona savignyi shown below. d. Ciona Hmx CNE analysis in Ciona.