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Quantification of BTN signal in control and Hmx CRISPR knockout embryos using the Ngn or Asic markers. Images in Fig. 2d.
| Readout | CRISPR control | Hmx CRISPR 6–10 | Chi squared (control vs. Hmx CRISPR | ||
|---|---|---|---|---|---|
| Embryo count | % with marked BTN | Embryo count | % with marked BTN | ||
| Foq>H2B:mCherry | 61 | 85% | 52 | 29% | 1.45E-07 |
| BTNs Ngn | 52 | 15 | |||
| Fog>H2B:mCherry | 43 | 84% | 50 | 34% | 8.33E-04 |
| BTNs Asic | 36 | 17 | |||
