Figure 2.

Systemic administration of adipolin ameliorates renal injury in WT mice after subtotal nephrectomy

(A) Urine and plasma parameters of renal function in WT mice treated with Ad-β-gal or Ad-APL at 8 weeks after subtotal nephrectomy. Left panel shows quantitative analysis of urinary albumin excretion normalized to urinary Cr. Center and right panels show plasma concentration of UN and Cr. N = 8 in each group.

(B) Evaluation of interstitial fibrosis in the injured kidney. Left panels show representative photos of the remnant kidney from Ad-β-gal-treated or Ad-APL-treated WT mice as determined by Masson Trichrome staining. Right panel shows quantitative analysis of fibrosis area as measured by ImageJ. N = 6 in each group. Scale bars show 100 μm.

(C) mRNA levels of pro-inflammatory mediators (TNFα, IL6, MCP1 and F4/80) in WT mice treated with Ad-β-gal or Ad-APL.

(D) mRNA levels of inflammasome-related genes (NLRP3, Caspase 1, IL1β and IL18) and oxidative stress markers (gp91^phox, p47^phox, p67^phox and p22^phox) in WT mice treated with Ad-β-gal or Ad-APL. N = 6 in each group. Student’s t-test ((A) (plasma UN), (B, C and D)) and Wilcoxon test ((A) (urine albumin and plasma Cr)) were used to produce the p values. N.S, not significant.