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. 2023 Apr 11;9(5):915–926. doi: 10.1021/acscentsci.2c01317

Figure 2.

Figure 2

Structure–activity relationship of CDK4 degrader. (a) Structures of EST1027 analogs assessing structure–activity relationships. (b) Testing EST1027 analogs in C33A cervical cancer cells to identify compounds that reduce CDK4 levels. C33A cells were treated with DMSO vehicle or compounds (5 μM) for 24 h. CDK4 and loading control GAPDH levels were assessed by Western blotting. (c) Full structure of hit compound EST1060. (d) Dose–response of EST1060 CDK4 degradation. C33A cells were treated with DMSO vehicle or EST1060 for 24 h. CDK4 and loading control GAPDH levels were assessed by Western blotting. Gels and blots in (b,d) are representative images from n = 3 biologically independent replicates/group.