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. 2023 Apr 24;9(5):980–991. doi: 10.1021/acscentsci.2c01325

Figure 2.

Figure 2

NS560 labels amino acids in HeLa cells. (A) Incubation of NS560 in HeLa cells for 45 min resulted in strong fluorescence at 5 μM. Scale bars, 40 μm. (B) Amino acid deprived HeLa cells incubated with NS560 showed increased labeling after replenishment with 5× essential amino acid solution. Scale bars, 30 μm. Representative images of three replicates. (C–D) Confocal microscopy reveals that NS560 signal can be surrounded by lysosomal marker Lamp1-RFP or dsRed-Rab7 under basal conditions. Scale bar, 5 μm. Representative images of three biological replicates.