Figure 4.

Chemical proteomics identifies lysosomal CQ targets. (A) Schematic of the proteomics strategy. Samples were prepared in triplicate for analysis. (B) Label-free proteomics results. Red bars indicate cut-offs (enrichment >2, chase rate >40%). (C) Relevant hits from proteomics data. (D) Validation of three chloroquine binding proteins from the proteomics data, CTSL, PSAP, and NPC2. CQ-X cross-linked targets were enriched via antibody and confirmed by blotting for cross-linked CQ-X via streptavidin blot. Representative Western blot for three biologically independent experiments.