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. 2023 Apr 24;9(5):980–991. doi: 10.1021/acscentsci.2c01325

Figure 5.

Figure 5

Chloroquine targets CTSL and NPC2 to regulate free amino acids. A549 cells were treated with shRNA for NPC2 for 48 h (A) or transfected with siRNA for CTSL for 48−72 h (C) then labeled with NS560 for 45 min. Representative Western blots from three biological replicates show efficient knockdown of all genes (B and D). (E) Representative quantification of NS560 foci per cell for images shown in C. (F) A549 cells treated with E64d (25 μM) or Pepstatin A (25 μM) for 24 h then labeled with NS560. Scale bar, 5 μm for all images. (G) Representative quantification of NS560 foci per cell for images shown in (F).