Fig. 2.

ROS scavenging ability of MF NPs. (A) Images of O₂ generation after 5 min of incubation with 2 mM H₂O₂ in different groups. (B) A time-dependent H₂O₂ decomposition assay performed over 5 min. (C) Michaelis − Menten kinetic analysis and (D) Lineweaver − Burk plot of the CAT-like activity of MF NPs with H₂O₂ as the substrate at 25 °C. (E) Michaelis − Menten constant (Km) and maximum reaction rate (Vmax) of commercial MnFe₂O₄, MF NPs, and CH6-MF NPs with H₂O₂ as the substrate. (F) XPS full-scan spectrum of MF NPs. (G) XPS high-resolution scans of C 1s of MF NPs. (H) XPS high-resolution scans of Mn 2p of MF NPs. (I) XPS high-resolution scans of Fe 2p of MF NPs. (J) Peroxidase (POD) activities of commercial MnFe₂O₄, MF NPs, and CH6-MF NPs at a concentration of 10 µg/ml. (K) Superoxide dismutase (SOD) activities of commercial MnFe₂O₄, MF NPs, and CH6-MF NPs at a concentration of 10 µg/ml. Student’s t test and one-way ANOVA were used to analyse the data. The outcomes are presented as the mean ± SD. ns = statistically nonsignificant, *P < 0.05, **P < 0.01, and ***P < 0.001; n = 3