TABLE 1.
Injected plasmid | No. of transformed lines showing excision | Total Neor transformed lines | % Excisiona |
---|---|---|---|
pIES-NEO | 8 | 10 | 80 |
pIES-72 | 13 | 15 | 87 |
pIES-31 | 2 | 10 | 20 |
pIES-18 | 6 | 14 | 43 |
pIES-1 | 0 | 15 | 0 |
Calculated by taking the number of transformed cell lines that contain some spliced 28-bp IES and dividing by the total number of transformed lines. Transformed lines showing excision varied in the amount of spliced product (10 to 100%).