Table 1.
Kinetic parameters of the interaction between the studied galectins and FGF4
| KD1 [M] | KD2 [M] | Kon1[M− 1 s− 1] | Kon2[M− 1 s− 1] | KOff1 [s− 1] | KOff2 [s− 1] | |
|---|---|---|---|---|---|---|
| gal-1 | 9.1E-07a | |||||
| gal-3 | 1.42E-07 | 1.07E-06 | 1.39E + 03 | 5.02E + 05 | 1.97E-04 | 5.38E-01 |
| gal-7 | 2.68E-07 | 4.97 E-07 | 1.21 E + 03 | 1.20E + 05 | 3.23E-04 | 5.96E-02 |
| gal-8 | 7.24E-07 | 1.40E-07 | 1.04E + 05 | 8.23E + 02 | 7.55E-02 | 1.15E-04 |
afor the interaction of galectin-1 with FGF4, as experimental data did not fit the kinetic models, steady-state analysis was used to determine Kd. Two values of KD, kon and koff were obtained from a 2:1 heterogenous ligand model, which was used to fit experimental kinetics data fitting