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. 2023 May 26;24(1):62. doi: 10.1186/s10194-023-01598-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Receptor autoradiography after estrogen treatment or withdrawal. Aspartate, AMPA, kainate, NMDA, and GABA_A binding sites were visualized by quantitative in vitro receptor autoradiography using D-[³H]aspartate, [³H]kainate, [³H]AMPA, [³H]MK-801 and [.³H]muscimol in frozen sections from ovariectomized female rats treated with vehicle (Ovx + V), with β-estradiol-3-benzoate (Ovx + E), or with β-estradiol-3-benzoate followed by withdrawal (Ovx + E/W). Specific radioligand binding in selected brain regions (superficial and deep cortical layers, in the striatum and in hippocampus (CA1, CA2, CA3) was assessed by measuring the optical density of the autoradiograms using a computerized image analysis system (Imaging Research, St. Catharines, Ontario, Canada). No significant treatment effect was noted for any of the radioligands. Individual values for each rat (expressed as Relative Optical Densities) are shown, together with group means and SEMs (n = 6 per treatment group)