Figure 1.

Osteoclast-osteomorph recycling in vitro

(A) The timeline of the experiment.

(B) Representative images of osteoclast fission. Red arrowheads indicate daughter cells.

(C) Screenshot of the key frame of Video S1. Red arrowheads indicate cell bridged process. Yellow arrowhead indicates daughter cell. Yellow dotted box indicates the cell outline.

(D) Screenshot of the key frame of Video S2. Red arrowheads indicate cell bridged process. Yellow arrowhead indicates daughter cell.

(E) Proportion of osteoclasts with different fates.

(F) The relative mRNA levels of DC-STAMP, TRAP, CTSK, and NFATc1 genes in bone marrow macrophages (BMMs), fused osteoclasts, and osteoclasts undergoing fission (n = 3).

(G) Western blot analysis of the protein levels of DC-STAMP, TRAP, CTSK, NFATc1, and GAPDH in BMMs, fused osteoclasts, and osteoclasts undergoing fission.

(H) The relative mRNA levels of the Fbxo7 and Bpgm genes in fused osteoclasts and osteoclasts undergoing fission (n = 3).

(I) Western blot analysis of the protein levels of Fbxo7, Bpgm, and GAPDH in fused and osteoclasts undergoing fission (n = 3).

(J) Quantification of Fbxo7 and Bpgm by immunoblotting.

∗p < 0.05 by Student’s t test (H and J) or by one-way ANOVA (F).