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. 2023 Apr 18;26(5):106682. doi: 10.1016/j.isci.2023.106682

Figure 4.

Figure 4

The alterations of mitochondria, lysosomes, and autolysosomes in osteoclast fission

(A) Representative MitoTracker and LysoTracker staining fluorescence images showing BMMs, fused osteoclasts, and osteoclasts undergoing fission (Mitochondria, green; lysosomes, red) (n = 5). Yellow arrowheads indicate that fluorescent signaling of MitoTracker and LysoTracker were merged in osteoclasts undergoing fission.

(B) Representative transmission electron microscope images showing BMMs, fused osteoclasts, and fission osteoclasts (n = 3). Red arrowheads indicate mitochondria. Yellow arrowheads indicate autolysosomes.

(C) Quantification of the proportion of the colocalization area to the total area in the image (n = 3).

(D) Quantification of mitochondria and autolysosomes by transmission electron microscope.

∗p < 0.05 by one-way ANOVA (C and D).