Figure 6.

Inhibiting mitophagy induces osteoclast apoptosis

(A) Western blot analysis of the protein levels of LC3B, p62, TOM20, BNIP3, PINK1, Parkin, and GAPDH in different osteoclast groups (n = 3). The osteoclasts were treated with DMSO, CQ, and Rap, respectively, after fused.

(B) Quantification of LC3B, p62, TOM20, BNIP3, PINK1, and Parkin by immunoblotting.

(C) Representative images of osteoclasts after treated with drugs 3 days (n = 3).

(D) The cell confluence in different group (n = 5).

∗p < 0.05 by one-way ANOVA (B).