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. 2023 Mar 2;21(6):1229–1239. doi: 10.1111/pbi.14032

Figure 3.

Figure 3

HSP90.2 positively regulates the photosynthesis rate and directly targets PsbO for its accumulation in the chloroplast. (a, b) The chlorophyll contents (a, n ≥ 20) and photosynthesis rates (b, n ≥ 10) in WT and hsp90.2 mutants. (c) The in vitro protein–protein interaction between PsbO and different domains of HSP90.2. (d) The binding between recombinant HSP90.2 C‐terminus and PsbO proteins. (e, f) The in vivo protein–protein interaction between full‐length HSP90.2 (e) and HSP90.2 C‐terminus (f) with PsbO. The WKS1.1 Interlinker START (IS) domain formed a homodimer and worked as the positive control. (g) The endogenous content of cytosolic HSP90 protein in the hsp90.2 knockout mutant. (h) The accumulation of PsbO in the chloroplasts of the hsp90.2 knockout mutant. (i) The endogenous content of cytosolic HSP90 protein in the HSP90.2 RNAi transgenic lines. (j) The accumulation of PsbO in the chloroplasts of the HSP90.2 RNAi transgenic lines.