Fig. 3.

Cqd1 is involved in mitochondrial phospholipid homeostasis. Strains were grown in synthetic medium containing glycerol (SCG) and mitochondria were purified by sucrose gradient centrifugation. Phospholipids were extracted and analyzed by mass spectrometry. The level of each phospholipid species (percentage of total phospholipids) is shown as a mean±s.d. of four biological replicates. *P≤0.05 (Mann–Whitney test). PC, phosphatidylcholine; PE, phosphatidylethanolamine; PS, phosphatidylserine; PI, phosphatidylinositol; CL, cardiolipin; PG, phosphatidylglycerol; PA, phosphatidic acid; MLCL, monolysocardiolipin. Relative amounts of different species of single phospholipids are presented in Table S2.