TABLE 1.

Analysis of crosses in which homing is associated with coconversion of flanking exon sequences^a

Cross	Donor allele	Recipient allele	Line no.	No. recb	Exon 1 markers				Exon 2 markers				Exon 3 markers				Type event
					E1−387 [Hpa+, Hpa−]	E1−20 [T, A]	E1−11 [G, A]	E1−6 [T, A]	E2−27 [T, C]	E2−8 [G, A]	E2−5 [T, (C)]	E2−2 [C, (T)]	E3+2 [C, (T)]	E3+5 [A, (G)]	E3+8 [A, (G)]	E3+23 [T, A]
A1	1o2+, wild-type	1o2o, Scap	1	2	D	D	D	D	D	D	*	*	*	*	*	R	RNA
			2	5	R	D	D	D	D	D	*	*	*	*	*	R	RNA
			3	2	D	D	D	D	D	D	*	*	*	*	*	D	DNA
			4	2	R	D	D	D	D	D	*	*	*	*	*	D	DNA
A2	1o2YAAA, RT mutant	1o2o, Scap	5	2	D	D	D	D	D	D	*	*	*	*	*	D	DNA
			6	7	R	D	D	D	D	D	*	*	*	*	*	D	DNA
			7	1	R	R	R	R	R	D	*	*	*	*	*	R	DNA
B	1o2+, wild-type	1o2o, E3+5G	8	3	D	D	D	D	D	D	*	*	*	R	*	R	RNA
			9	6	R	D	D	D	D	D	*	*	*	R	*	R	RNA
			10	3	D	D	D	D	D	D	*	*	*	D	*	D	DNA
			11	8	R	D	D	D	D	D	*	*	*	D	*	D	DNA
C	1o2+, wild-type	1o2o, E3+8G	12	6	R	D	D	D	D	D	*	*	*	*	R	R	RNA
			13	2	R	R	D	D	D	D	*	*	*	*	R	R	RNA
			14	2	D	D	D	D	D	D	*	*	*	*	D	D	DNA
			15	7	R	D	D	D	D	D	*	*	*	*	D	D	DNA
			16	1	R	R	R	R	R	R	*	*	*	*	D	D	DNA
			17	1	R	D	D	D	D	D	*	*	*	*	D	R	DNA
D	1o2+, wild-type	1o2o, E2−2T−5C E3+2T+5G	18	4	D	D	D	D	D	D	D	D	R	R	*	R	RNA
			19	14	R	D	D	D	D	D	D	D	R	R	*	R	RNA
			20	1	R	R	D	D	D	D	D	D	R	R	*	R	RNA
			21	2	R	R	R	R	D	D	D	D	R	R	*	R	RNA
			22	10	D	D	D	D	D	D	D	D	D	D	*	D	DNA
			23	7	R	D	D	D	D	D	D	D	D	D	*	D	DNA
			24	1	R	R	D	D	D	D	D	D	D	D	*	D	DNA
			25	1	R	D	D	D	D	D	D	D	D	D	*	R	DNA

^aRecombinant progeny were isolated from each cross as described in Materials and Methods. For each recombinant strain the exons flanking the aI2 insertion site were sequenced so that all of the flanking markers present in each cross were scored (see also Fig. 1). The nucleotide present at each site in donor and recipient strains is shown in brackets above each column as follows: [donor, recipient]. Alleles in parentheses are present only in some of the recipient strains, as indicated. Asterisks indicate that the donor and recipient strains in that cross have the same nucleotide. For each cross, results are organized according to the type of event that likely led to each coconversion pattern. Coconversion tracts are indicated in boldface type. In the type event column, “RNA” denotes the products of retrohoming, while “DNA” denotes the products of RT-independent homing. The rationale for these assignments is presented in the text. Figure 5 illustrates some of these patterns schematically. D, donor allele (intron insertion was accompanied by coconversion at that site); R, recipient allele (intron insertion was not accompanied by coconversion at that site). 

^bNo. rec, number of recombinant progeny analyzed for flanking markers.