Skip to main content
. Author manuscript; available in PMC: 2023 Oct 1.
Published in final edited form as: Biochim Biophys Acta Mol Basis Dis. 2022 Jun 13;1868(10):166456. doi: 10.1016/j.bbadis.2022.166456

Fig. 9.

Fig. 9.

Effects of arachidonic acid (AA) or AEA-COX-2 substrate-selective inhibitor on protective effects of FAAH inactivation on TGF-β1 induced-fibrogenesis in HK-2 cells. (A-B) Representative immunoblots and quantitative data of α-SMA in HK-2 cells with different combinations of treatments with TGF-β1, arachidonic acid (AA, 10 μM) and FAAH inhibitor PF-04457845 (PF, 1 μM). (C–D) Representative immunoblots and quantitative data of α-SMA and collagen I/III expressions in HK-2 cells with different combinations of treatments with TGF-β1, AEA-COX-2 pathway substrate-selective inhibitor (LM-4131, 30 nM), and PF. (E-F) Representative immunoblots and quantitative data of α-SMA expression in HK-2 cells with prostamide E2 (100nM) and TGF-β1. All values were normalized to Ctrl group and shown as mean ± SEM. *P < 0.05, **P < 0.01, ****P < 0.0001 by one-way ANOVA Bonferroni’s multiple comparisons test. Ctrl: control. ns: not significant.