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. 2023 May 13;12(5):1097. doi: 10.3390/antiox12051097

Figure 4.

Figure 4

Effect of SC on high fat diet-induced hepatic injury in vivo and antioxidant-related proteins in vitro. (A) H&E images of liver tissue from a representative mouse in each group (scale bar represents 100 μm). Mice were fed as described in Figure 2A. (B) Blood markers of liver injury. The levels of aspartate aminotransferase were measured in the blood serum of mice from the respective group. (C) mRNA expression of NOX4 in liver tissue. The extracted mRNA from mouse livers in the respective groups was analyzed by quantitative real-time PCR. Statistical significance of aspartate aminotransferase levels in plasma and NOX4 mRNA levels in liver tissue was indicated by comparison with the ND-fed group (* p < 0.05) and HFD-fed group (# p < 0.05). (D) Immunoblotting analysis of Nrf2 in HepG2 cell nucleus. HepG2 cells were incubated in a serum-free media for 12 h, followed by treatment with 100 μg/mL of SC for 3, 6, and 12 h. Lamin A/C are loading controls of nucleus proteins for immunoblotting analysis. (D) Immunoblotting analysis of the expression HO-1 by SC. HepG2 cells proceeded as described in Figure 4D.