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(A) Immunoblots for protein expression of GCLC, GCLM and GSS. Each blot represents 3 sets from the brains of young mice (Y), old-control mice (OC) and old mice on GlyNAC supplementation (OG). GCLC and GCLM = Glutamate cysteine ligase, catalytic (GCLC) and modifier (GCLM) subunits; GSS = Glutathione Synthetase. (B–D) Immunoblot quantification: optical density of protein expression was normalized to the loading control (β-actin). ∗ = p < 0.05; φ = p < 0.01, θ = p < 0.001.
