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. 2023 Apr 24;12(5):986. doi: 10.3390/antiox12050986

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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RPLC-MS/MS analysis and annotation of oxidized PC 16:0_18:2;O2 isomers. (a) Extracted ion chromatograms (EIC) in ESI(−) for the precursor ion m/z 848.5658, [M+CH₃COO]^– in dry serum extracts (red EIC representing analysis of dry extract stored at −80 °C, blue EIC representing analysis of dry extract stored at +30 °C); (b) HR-MS/MS product ion spectrum of PC 16:0_18:2;O2 in ESI(−) eluted at 1.07 min; (c) HR-MS/MS product ion spectrum of PC 16:0_18:2;O2 in ESI(−) eluted at 1.16 min; (d) EIC in ESI(+) for the precursor ion m/z 790.5598, [M+H]⁺ in dry serum extracts (red EIC representing analysis of dry extract stored at −80 °C, blue EIC representing analysis of dry extract stored at +30 °C); (e) HR-MS/MS product ion spectrum of PC 16:0_18:2;O2 in ESI(+) eluted at 1.07 min; (f) HR-MS/MS product ion spectrum of PC 16:0_18:2;O2 in ESI(+) eluted at 1.17 min.