Effects of MG infection on TLRs gene expressions in chicken embryos and chicks. (a) The mRNA expression level of TLRs in lungs of chicken embryos after MG infection by RNA-seq. (b) The mRNA expression level of TLRs in lungs of chicken embryos after MG infection by RT-qPCR. (c) The mRNA expression level of TLRs in lungs of chicks after MG infection by RNA-seq. (d) The mRNA expression level of TLRs in lungs of chicks after MG infection by RT-qPCR. The mRNA expression level of TLRs in the spleen (e), thymus(f), and bursa of Fabricius (g) of chicken embryos after MG infection by RT-qPCR. The mRNA expression level of TLRs in the spleen (h), thymus (i), and bursa of Fabricius (j) of chicks after MG infection by RT-qPCR. GAPDH was used as a control. The data were represented as the mean ± SD. n ≥ 4. * p < 0.05; ** p < 0.01, *** p < 0.001.