Figure 4.

Biomimetic BBB-on-chip models compartmentalized by a membrane and hydrogel. (A) Schematic representation of the four-cell model developed by Brown et al. [62] with a polycarbonate membrane and a collagen I hydrogel (top panel), and a photograph of the assembled device loaded with colored dyes indicating different compartments: red = vasculature; semi-transparent white  =  filter membrane; turquoise = brain compartment; and blue = brain perfusion (bottom panel). OA publishing license: Adapted from Brown et al. [62]. (B) Schematic representation of the four-cell model developed by Koo et al. [76] with a collagen I hydrogel (top panel) and structure and format application of in vitro cultureware, OrganoPlate consisting of a 384-well microliter plate with two lanes (bottom panel). OA publishing license: Adapted from Koo et al. [76].