Figure 1.

Schematic representation of the OMVs in this study. PTE or DFPase was expressed as a fusion protein with various anchors/directors, including to the anchor Lpp’ with increasing linker length (Lpp’, L3, L4, and L34), a truncated form of transmembrane porin protein (OmpA) as an anchor fused to SpyTag (ST) that binds PTE/DFPase fused to SpyCatcher (SC), PTE/DFPase-SC as a non-directed control, to periplasmic proteins as directors including maltose-binding protein (MBP) and vitamin B12-binding protein (BtuF), and to two additional lipoprotein anchors SLP and SlyB.