Figure 1.

Effect of storage time on cell quality and differentiation potential. Before transplantation, human bone-marrow-derived mesenchymal stem cells (hBM-MSCs), which were expanded ex vivo and detached from the culture dish, were analyzed to evaluate their quality and differentiation potential. The transcriptome [58] and metabolome [57] were analyzed using microarray and gas chromatography-mass spectrometry (GC-MS), respectively. By integrating these datasets with in silico prediction, it was found that as the quality and differentiation potential of hBM-MSCs decreased and ROS production, lipid peroxidation, and cell damage increased in phosphate-buffered saline (PBS) over time.