Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Schematic protocol for building a 3D organotypic model of pleura and its infection with bacteria. HPFs were mixed with collagen type I (A) to generate layer 1 (B). Layer 1 was polymerized and HPMCs were placed on top to build layer 2, solidifying for 24 h (C). Then, 24 h before bacterial inoculation, the medium was changed to PBS with BSA (1%) without antibiotics (D). Infection of 3D co-culture model with S. aureus was induced (E).
