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Biofilms of S. aureus (ATCC 49230) in 3D co-culture model monitored over 24 h. S. aureus colony-forming units (CFUs) within target sites of infection after inoculation over time. The 3D co-culture model was inoculated with S. aureus: ● = 103 CFU/mL, ■ = 104 CFU/ml, ▲ = 105 CFU/ml, ▼ = 106 CFU/ml. Bacterial burden was measured after 3 h, 6 h, 12 h, and 24 h. P values represent results for ANOVA test comparing CFUs at 0 and 24 h (*** p = 0.002). N = 6, error bars indicate SD.
