Table 1.

Temporal development of biofilm on the 3D co-culture model of pleura.

Incubation Period (Hours)	Group B: S. aureus (1 × 103 CFU/mL)	Group C: S. aureus (1 × 106 CFU/mL)
0	no bacteria	no bacteria
3	single bacterial cells	bacterial micro-colonies
6	bacterial micro-colonies	bacterial aggregates
12	bacterial aggregates in tissue, biofilm	mature biofilm
24	mature biofilm	mature biofilm