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. 2023 May 11;12(5):1083. doi: 10.3390/antiox12051083

Figure 2.

Figure 2

OxLDL increases high-glucose-dependent inflammatory responses in macrophages. (A,B) THP1 MΦ or MDM were treated with 150 μg/mL of oxLDL for 72 h in culture medium with 5 mmol/L (normal glucose, NG) or 15 mmol/L (high glucose, HG) of glucose. Cells were also stimulated with 10 ng/mL of LPS in NG. Culture supernatants were collected, and the amount of TNF (A), IL1B, IL8, MCP1, and IL10 (B) was analyzed by ELISA. Data from at least 4 independent experiments for THP1 MΦ or 4 donors for MDM, performed in triplicate, are shown. (C) THP1 cells were left untreated (-), transfected with siRNA targeting CD36 (CD36), or transfected with a non-targeting negative control (Ct), incubated with oxLDL under HG conditions for 24 h. CD36 surface expression was analyzed by flow cytometry, and TNF in culture supernatants was measured by ELISA. The graph shows the mean ± SEM from 3 independent experiments performed in triplicate (* p < 0.05, ** p < 0.01, *** p < 0.001, Mann–Whitney test). MDM: monocyte-derived macrophages. iMFI: integrated mean fluorescence intensity.