Table 4.
Effects of different brown and green seaweed levels on plasma antioxidant enzyme activities in broiler chickens.
Enzymes 1 | Dietary Treatments 2 | SEM 3 | p-Values | Contrast p-Values 4 |
||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NC | PC | BS 0.25 | BS 0.50 | BS 0.75 | BS 1 | BS 1.25 | GS 0.25 | GS 0.50 | GS 0.75 | GS 1 | GS 1.25 | Line. | Quad. | |||
CAT (U/L) | 4.11 | 3.71 | 3.73 | 3.73 | 3.65 | 3.84 | 4.16 | 3.46 | 3.97 | 3.96 | 3.92 | 4.00 | 0.21 | 0.6222 | 0.3072 | 0.1506 |
SOD (U/mL) | 3.10 | 3.11 | 3.14 | 3.08 | 3.11 | 3.09 | 3.15 | 3.04 | 3.10 | 3.12 | 3.14 | 3.11 | 0.04 | 0.9395 | 0.4899 | 0.8876 |
GPx (U/L) | 0.48 | 0.48 | 0.51 | 0.46 | 0.49 | 0.47 | 0.43 | 0.51 | 0.44 | 0.46 | 0.46 | 0.47 | 0.03 | 0.6493 | 0.5120 | 0.6833 |
1 Enzymes: CAT = catalase. One unit is the amount of CAT that decomposes 1 µmole of H2O2 per min at pH 7.0 and room temperature; SOD = superoxide dismutase. One unit is the amount of SOD that catalyze 1 µmole of superoxide into O2 and H2O2 per min under the condition of the assay; GPx = glutathione peroxidase. One unit is the amount of GPX that produces 1 µmole of glutathione disulfide per min at pH 7.6 and room temperature. 2 Dietary treatments: NC (negative control) = basal diet, PC (positive control) = basal diet + vitamin E (100 mg/kg feed), BS 0.25 = basal diet + 0.25% brown seaweed, BS 0.50 = basal diet + 0.50% brown seaweed, BS 0.75 = basal diet + 0.75% brown seaweed, BS 1 = basal diet + 1% brown seaweed, BS 1.25 = basal diet + 1.25% brown seaweed, GS 0.25 = basal diet + 0.25% green seaweed, GS 0.50 = basal diet + 0.50% green seaweed, GS 0.75 = basal diet + 0.75% green seaweed, GS 1 = basal diet + 1% green seaweed, GS 1.25 = basal diet + 1.25% green seaweed. 3 SEM = standard error of means. 4 Contrast p-Values = orthogonal polynomial contrasts of dietary increasing brown and green seaweed inclusion levels (0.0 to 1.25%).