TABLE 1.
Yeast strains used in this study
| Strain | Genotype | Reference or sourcea |
|---|---|---|
| C276-4A | MATa gal2 | 39 |
| DJTD2-16A | MATa cdc42-1 his4 leu2 trp1 ura3 | 17 |
| PMYD9-1B | MATa ura3-52:cdc42W97R:URA3 Δcdc42::TRP1 trp1-Δ101 leu2 ade2-101lys2-801 his | 26 |
| EGY48-p1840 | MATα ura3 his3 trp1 integrated lexAop-LEU2 integrated lexAop-lacZ | 11 |
| DJD6-11 | MATa/MATα Δcdc42::TRP1/+ his3Δ200/+ his4/+ leu2/+ can1/+ lys2-801/lys2-801 trp1-Δ1/trp1-Δ101 ade2-101/+ ura3-52/ura3-52 | 26 |
| TRY11-7D | MATα leu2 ura3 trp1 his | 33 |
| DLY1028 | MATa Δswe1::LEU2 ade2 ura3 trp1 his2 | 33 |
| TRY13-5A | MATa his3 leu2 ura3 trp1 | This study |
| TRY32 | MATa/MATα Δcdc42::TRP1/+ ura3-52:cdc42Y32K:URA3/ura3-52 leu2/+ lys2-801/lys2-801 his3Δ200/+ his4/+ trp1-Δ1/trp1-Δ101 ade2-101/+ can1/+ | This study |
| TRY37 | MATa/MATα Δcdc42::TRP1/+ ura3-52:cdc42F37A:URA3/ura3-52 leu2/+ lys2-801/lys2-801 his3Δ200/+ his4/+ trp1-Δ1/trp1-Δ101 ade2-101/+ can1/+ | This study |
| TRY38 | MATa/MATα Δcdc42::TRP1/+ ura3-52:cdc42D38E:URA3/ura3-52 leu2/+ lys2-801/lys2-801 his3Δ200/+ his4/+ trp1-Δ1/trp1-Δ101 ade2-101/+ can1/+ | This study |
| TRY38-2B | MATa Δcdc42::TRP1 ura3-52:cdc42D38E:URA3 leu2 lys2-801 his3Δ200 trp1Δ1 | This study |
| TRY38-8A | MATα Δcdc42::TRP1 ura3-52:cdc42D38E:URA3 leu2 lys2-801 his3Δ200 his4 trp1Δ1 ade2-101 | This study |
| TRY39 | MATa/MATα Δcdc42::TRP1/+ ura3:cdc42D38E:URA3/ura3 ade2-101/ade2 Δswe1::LEU2/+ leu2/leu2 trp1-Δ101/trp1 his2/+ his3/+ his4/+ lys2-801/+ | This study |
| TRY40 | MATa/MATα Δcdc42::TRP1/+ ura3-52:cdc42Y40C:URA3/ura3-52 leu2/+ lys2-801/lys2-801 his3Δ200/+ his4/+ trp1-Δ1/trp1-Δ101 ade2-101/+ can1/+ | This study |
| TRY41A | MATa/MATα Δcdc42::TRP1/+ ura3-52:cdc42D38E:URA3/ura3 leu2/leu2 his3/his3 trp1Δ1/trp1 ade2/ade2 +/Δcla4::trp1::HIS3 | This study |
| TRY46 | MATa/MATα Δcdc42::TRP1/+ ura3-52:cdc42D38E:URA3/ura3 +/ade1 leu2/leu2 trp1/trp1 Δbem3::LEU2/+ his3/his3 lys2-801/+ | This study |
| TRY48 | MATa/MATα ade1/++/ade2 ura3/ura3 leu2/leu2-3 his3/his3 trp1/trp1 +/Δcla4::trp1::HIS3 bem3::LEU2/+ | This study |
a
TRY32 was generated by integrating linearized pRS306(cdc42Y32K) into DJD6-11. TRY37, TRY38, and TRY40 were created in the same manner as TRY32 with pRS306(cdc42F37A), pRS306(cdc42D38E), and pRS306(cdc42Y40C), respectively. TRY39 was generated by crossing TRY38-8A with DLY1028. TRY41A was generated by crossing TRY38-2B with TRY41-3A (a Δcla4::trp1::HIS3 strain generated by tetrad dissection of TRY41, which was generated by crossing TRY38-8B with TRY3-H [33]). TRY46 was generated by crossing TRY38-2B with TRY4-1B (a Δbem3::LEU2 strain generated by tetrad dissection of SY3032 [37]). TRY48 was generated by crossing TRY3-H with TRY4-1B.