TABLE 4.
Functionality of effector domain mutant allelesa
| Mutation | Growth
|
||
|---|---|---|---|
| cdc42-1ts, 37°C | cdc42W97R, 37°C | Δcdc42, 23°C | |
| None (vector) | − | − | − |
| CDC42 | + | + | + |
| Y32K | ± | − | − |
| F37A | ± | − | − |
| D38E | + | ± | + |
| Y40C | + | − | − |
| V44A | + | + | + |
a
See Materials and Methods for a description of the complementation of the Δcdc42 mutant. pRS315(CDC42), pRS315(cdc42Y32K), pRS315(cdc42F37A), pRS315(cdc42D38E), pRS315(cdc42Y40C), and pRS315(cdc42V44A) were transformed into strains DJTD2-16A (cdc42-1 ts) and PMYD9-1B (cdc42W97R ts) and selected on SC-Leu medium. Transformants were then streaked on SC-Leu medium at 23 and 37°C to test for viability at restrictive temperatures. −, no growth; ±, partial growth; +, normal growth.