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Standard curve of the TRFIA. (A) Standard curves of TRFICA for DNC in an assay buffer under optimized conditions. (B) Fluorescent image of the TRFICA strip acquired under ultraviolet light for 0, 0.02, 0.06, 0.19, 0.57, 1.67, 5.0, and 15 ng mL−1 in an assay buffer (left to right). (C) Comparison of the TRFICA curves obtained from the standards prepared in the assay buffer and the 20-fold diluted chicken extracts.
