Figure 11.

(a) Relative change in the total number of cancer cells for 5 responder (R) cases and 5 non-responder (NR) cases. For each case, the solid line with different patterns is the mean of 10 replications, and the band displays the standard deviation (SD). Among R cases, the final number of cancer cells is smaller in R1, R3 and R5 with respect to R2 and R4. NR3, NR5, NR2, NR4 and NR1 have the largest final number of cancer cells among NR cases. (b) Cell distribution in the center-cropped ROI for R cases before treatment (BT). Blue: cancer cells; green: CD8+ T cells; red: regulatory T cells. In R1, R3 and R5, the ratio of cancer cells to immune cells is smaller. (c) Cell distribution in the center-cropped ROI for cases after treatment (AT). R1, R3 and R5 demonstrate more obvious compartmentalized patterns because they show better response to the therapy. (d) Cell distribution in the center-cropped ROI for NR cases before treatment (BT). NR1 and NR4 have more mixed structure before treatment. (e) Cell distribution in the center-cropped ROI for NR cases after treatment (AT). The tumor-immune patterns do not change significantly for NR cases.