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. 2023 May 8;11(5):1400. doi: 10.3390/biomedicines11051400

Figure 2.

Figure 2

FBEVs contain pro-angiogenic cytokines and activate MMECs. (A) FBEVs were lysed and analyzed for cytokine cargo using an angiogenic array. Data are expressed as pixel density, normalized to reference spots, and expressed as mean ± S.D. (B) Representative flow cytometry analysis of MMECs co-cultured with FBEVs for 1 h and analyzed for phospho-VEGFR2, phospho-HGFR, and phospho-Tie2 expression by flow cytometry. (C) Analysis of cell sprouting of MMECs cultured with/without FBEVs and/or (D) pre-treated with blocking antibodies against VEGFR2, HGFR, and Tie2. Representative images of three independent experiments of Matrigel®-based 3D culture are shown. Scale bar = 25 μm. (E) Bar graphs indicate the percentage of cell spreading/field of MMECs cultured with/without FBEVs and/or pre-treated with blocking antibodies against VEGFR2, HGFR, and Tie2. Data are expressed as mean ± S.D. * p < 0.05, *** p < 0.001.